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Microchip for scientific research

BiomicrochipSemiotik-array.com.jpg

The array is represented by mammalian glycans and bacterial polysaccharides.

The following is included in the delivery set for research from Semiotik:

  • microchips;
  • a set of ready reagents (on demand); 
  • fluorescence reader (on demand);

Use of research microchip:

  • search of glycan-binding proteins and characterization of their specificity; the proteins are: antibodies, lectins, glycosyltransferases, glycosidases [1-7];
  • functional revealing of lectins and other carohydrate-binding and carbohydrate-modifying molecules in composition of bacteria, viruses and cells;
  • development of anti-bacterials and anti-virals acting on microorganism’s lectins;
  • search of diagnostic signatures (combination of several antibodies-markers), for cancer, autoimmune and other diseases diagnostics [8-11].

The chip parameters:

  • 25 х 75 mm size, each microchip has a bar code; 
  • Time needed for typical analysis is ~3 hr;
  • About 400 glycans, 200 bacterial polysaccharides, peptides, proteins are immobilized, all in 6 replicates;
  • Specific IgG and IgM are measured separately;
  • Material: glass or plastic;
  • Shelf life is 1 year at a temperature +4°C.

Advantages of microchip use for research: 

  • decrease of studies duration compared to standard ELISA;optimization of costs of immunological studies;

  • personnel costs decrease for performing of a scientific task;

  • standardization of screening studies; 

  • revealing of new scientifically significant antibodies;

  • there is no need in repeats/controls of each sample because every microchip contains internal control/repeat of each spot.

 Data processing:

  • the titer of bound antibodies, i.e. relative content of antibodies in a sample of blood/plasma is determined by the spot fluorescence intensity using a fluorescence reader;
  • the protein in test should possess a direct fluorescent label, an alternative is labeled antibody against the protein;
  • the results of readout are converted into digital format and decoded using software, available for registered users (release in 2016)
  • mathematical tools for the search of diagnostic signatures are described in [10].

Equipment necessary for the work with chips